Combining the science of genetics with the business of discovery, we are moving de-extinction forward by resurrecting the Woolly Mammoth through gene editing.
About the role
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What they're looking for
- Expert knowledge of primary cell derivation, culture, propagation, and biobanking
- Expert knowledge and hands-on experience in CRISPR gene editing, including proficiency with multiple gene delivery methods such as chemical transfection, electroporation, and LNP delivery
- Experience with flow cytometry, immunostaining, confocal microscopy
- Microdissection experience
- Strong Aseptic Technique and ability to work with antibiotic free culture
- Strong written and oral communication skills, as well as creative problem-solving skills
More about this role
An affiliate of Colossal is hiring a Scientist that will derive non-model organism primary cell lines, conduct assays corresponding to cell health, characterize lines, perform transfections, and deliver edited lines for downstream SCNT. The candidate will be part of a core team responsible for optimizing and characterizing for lines derived in house and supporting other parts of the cell core team in gene editing workflows. This position reports to the Manager of Primary Cell and Production sub-team within the Cell Engineering and Production (CEP) core team.
This position is based out of Dallas, TX. Relocation assistance is available.
- Isolate, expand, and maintain high-quality primary cell cultures from diverse non-model animal tissues.
- Innovate then execute experiments to generate and characterize primary cells from non-model mammalian organisms, develop functional assays, assess and interpret experimental workflows
- Work with team members to assess efficiency of workflows through cellular assays and NGS sequencing approaches
- Work closely with genome engineers to screen, validate, and make cell lines that will undergo SCNT
- Work on project milestones
- Attend lab...
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