Are you ready to play a key role in Gordian’s discovery pipeline by producing the AAV vectors that power every one of our in vivo screens? You will operate as a key member of the team producing AAV for all of Gordian, reliably executing laboratory protocols,... Backed by Stanford.
About the role
Your primary mission as Vector Engineering research associate is to play a key role in the execution and workflows responsible for producing AAV for all of Gordian. You’ll contribute to the production of AAV vectors through adherent and suspension HEK293 cell transfection techniques and a suite of scalable AAV purification techniques (iodixanol ultracentrifugation, depth filtration, TFF, FPLC). Found on 1752vc Careers, the job board for startup and VC roles.
What they're looking for
- Tissue culture experience
- Wet lab experience such as transfections, qPCR, Western blot, or SDS-PAGE
- Experience with flow systems such as FPLC, TFF, or depth filtration
- Previous experience with AAV, lentivirus, or gene therapy
More about this role
Your primary mission as Vector Engineering research associate is to play a key role in the execution and workflows responsible for producing AAV for all of Gordian. You’ll contribute to the production of AAV vectors through adherent and suspension HEK293 cell transfection techniques and a suite of scalable AAV purification techniques (iodixanol ultracentrifugation, depth filtration, TFF, FPLC).
In your first month, you will shadow team members to learn the Vector team’s adherent and suspension production protocols, documentation standards, and workflows. You will take on routine lab tasks such as media prep and media changes with care and attention to detail, with no labeling errors or sample swaps and clear, detailed records in our ELN. You will learn how the Vector team interfaces with the groups that request AAV, and help our HFpEF and CKD programs by producing critical AAV vector.
Within three months, you will independently run core production tasks such as HEK293 cell splits, transfections, and harvests, and perform purification steps such as iodixanol ultracentrifugation and FPLC with limited supervision. You will have expanded your understanding of AAV biology, particularly...
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